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nuclei lysis buffer  (Miltenyi Biotec)


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    Miltenyi Biotec nuclei lysis buffer
    Nuclei Lysis Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/extraction+buffer/Nuclei+Extraction+Buffer/pmc13378589-245-8-14
    Average 96 stars, based on 112 article reviews
    nuclei lysis buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Extraction:

    Article Title: Targeting angiopoietin like-2 positive senescent cells improves cognitive impairment in adult male but not female atherosclerotic LDLr -/- ;hApoB 100 +/+ mice.
    Article Snippet: They were isolated by combining enzymatic treatment with gentle mechanical dissociation using GentleMACS Octo Dissociator (Miltenyi Biotec, Gaithersburg, MD, USA). .. Briefly, frozen segments of hippocampus were put into the Nuclei Vol:. (1234567890) Extraction Buffer (#130–128-024, Miltenyi Biotec, Gaithersburg, MD, USA) in C-Tube and processed for 5 min. After two steps of washing and filtration (100 and 70 μm + 20 μm stack together, respectively), nuclei were counted both automatically (CountessTM II Automated Cell Counter) and manually (hemacytometer). ..

    Article Title: Time series single-cell transcriptional atlases reveal cell fate differentiation driven by light in Arabidopsis seedlings.
    Article Snippet: .. For single-nucleus data, the shoot tissues of dark-grown seedlings were frozen in liquid nitrogen, added to nuclear extraction buffer, fragmented with a gentleMACS Octo dissociator (Miltenyi Biotec, 130- 095-937), and finally filtered and suspended in 300 μl of 10x wash buffer for nuclear isolation67. .. The protoplast or nuclear suspension was loaded into Chromium microfluidic chips with v.3.1 chemistry and barcoded with a Chromium Controller (10x Genomics).

    Article Title: BDNF gene delivery to the retina by cell adhesion peptide-conjugated gemini nanoplexes in vivo.
    Article Snippet: Retinal ganglion cell (RGC) neurodegeneration in glaucoma is not prevented by controlling the elevated intraocular pressure alone.. Neuroprotective gene therapy approaches could be an essential part of a combination treatment.. Five cell adhesion peptide (CAP)-gemini surfactants (18-7N(p1–5)-18) were synthesized as building blocks for brain-derived neurotrophic factor (BDNF) gene carrier nanoparticles (CAP-NPXs).

    Article Title: SIRT1 Ameliorates Lamin A/C Deficiency-Induced Cardiac Dysfunction by Promoting Mitochondrial Bioenergetics
    Article Snippet: .. Sample preparation: Tissues were homogenized separately in 400 μL lysis buffer containing RIPA and Extraction Buffer, protease inhibitor, phosphatase inhibitor and 8 M urea using the gentleMACS Dissociator (Miltenyi Biotec). .. Extracted proteins were reduced and alkylated respectively with 10 mM dithiothreitol (Sigma-Aldrich, #D0632) and 15 mM iodoacetamide (Sangon Biotech, #A600539).

    Article Title: The Arabidopsis homolog of microspherule protein 1 is essential for embryogenesis and interacts with the Myb-like transcription factor DRMY1.
    Article Snippet: The evolutionarily conserved microspherule protein 1 (MCRS1) has diverse functions, ranging from transcriptional regulation to stabilization of microtubule minus ends in acentrosomal spindles in mammals.. A previous study suggested that in the model plant Arabidopsis thaliana, inactivation of an MCRS1 homolog gene led to aborted embryogenesis.. To test whether this lethality was caused solely by sporophytic defects, we used the heterozygous emb1967-1/mcrs1-1 mutant for reciprocal crosses with the wild-type plant and found that the MCRS1 gene was dispensable for the development of both male and female gametophytes.

    Article Title: Functional analogs of mammalian 4E-BPs reveal a role for TOR in global plant translation.
    Article Snippet: .. 1 g 10-day old 35S::HF-RPL18 seedlings were homogenized by grinding in 2 mL extraction buffer (200 mM Tris-HCl pH9.0, 200 mM KCl, 25mMEGTA pH 8.0, 35mMMgCl2, 0.05%DOC, 0.05%PTE, 0.05%BriJ- 35, 0.05%Triton X-100, 0.05% Igepal, 0.05%Tween 20, 10 mM DTT, 10 mM Mg132, protease inhibitor EDTA-free) at 4 C for 10 to 15 min before centrifugation at 16,000 g for 10 min at 4 C. The clear supernatant was incubated with 50 mL of mMACSTM Anti-Tag MicroBeads (Miltenyi Biotec, DYKDDDDK Isolation Kit: 130-101-591) for 30 min at 4 C and then loaded in a mMACSTM column. ..

    Article Title: Functional analogs of mammalian 4E-BPs reveal a role for TOR in global plant translation.
    Article Snippet: .. Myc-4EBP2 IP 1 g 10-day old 4EBP2ox seedlings were homogenized by grinding in 2 mL extraction buffer (50 mM Tris-HCL, pH 7.5; 100 mMNaCL, 5% Glycerol, 0.05% Tween 20) at 4 C for 10 to 15 min before centrifugation at max speed for 10 min at 4 C. The clear supernatant was incubated with 50 mL of c-myc micro-beads (Miltenyi Biotec, mMACSTM c-myc Isolation Kit: 130-091-123) for 30 min at 4 C and then loaded in a mMACSTM column. ..

    Article Title: Time series single-cell transcriptional atlases reveal cell fate differentiation driven by light in Arabidopsis seedlings
    Article Snippet: .. For single-nucleus data, the shoot tissues of dark-grown seedlings were frozen in liquid nitrogen, added to nuclear extraction buffer, fragmented with a gentleMACS Octo dissociator (Miltenyi Biotec, 130-095-937), and finally filtered and suspended in 300 μl of 10x wash buffer for nuclear isolation . .. The protoplast or nuclear suspension was loaded into Chromium microfluidic chips with v.3.1 chemistry and barcoded with a Chromium Controller (10x Genomics).

    Filtration:

    Article Title: Targeting angiopoietin like-2 positive senescent cells improves cognitive impairment in adult male but not female atherosclerotic LDLr -/- ;hApoB 100 +/+ mice.
    Article Snippet: They were isolated by combining enzymatic treatment with gentle mechanical dissociation using GentleMACS Octo Dissociator (Miltenyi Biotec, Gaithersburg, MD, USA). .. Briefly, frozen segments of hippocampus were put into the Nuclei Vol:. (1234567890) Extraction Buffer (#130–128-024, Miltenyi Biotec, Gaithersburg, MD, USA) in C-Tube and processed for 5 min. After two steps of washing and filtration (100 and 70 μm + 20 μm stack together, respectively), nuclei were counted both automatically (CountessTM II Automated Cell Counter) and manually (hemacytometer). ..

    Sample Prep:

    Article Title: SIRT1 Ameliorates Lamin A/C Deficiency-Induced Cardiac Dysfunction by Promoting Mitochondrial Bioenergetics
    Article Snippet: .. Sample preparation: Tissues were homogenized separately in 400 μL lysis buffer containing RIPA and Extraction Buffer, protease inhibitor, phosphatase inhibitor and 8 M urea using the gentleMACS Dissociator (Miltenyi Biotec). .. Extracted proteins were reduced and alkylated respectively with 10 mM dithiothreitol (Sigma-Aldrich, #D0632) and 15 mM iodoacetamide (Sangon Biotech, #A600539).

    Lysis:

    Article Title: SIRT1 Ameliorates Lamin A/C Deficiency-Induced Cardiac Dysfunction by Promoting Mitochondrial Bioenergetics
    Article Snippet: .. Sample preparation: Tissues were homogenized separately in 400 μL lysis buffer containing RIPA and Extraction Buffer, protease inhibitor, phosphatase inhibitor and 8 M urea using the gentleMACS Dissociator (Miltenyi Biotec). .. Extracted proteins were reduced and alkylated respectively with 10 mM dithiothreitol (Sigma-Aldrich, #D0632) and 15 mM iodoacetamide (Sangon Biotech, #A600539).

    Protease Inhibitor:

    Article Title: SIRT1 Ameliorates Lamin A/C Deficiency-Induced Cardiac Dysfunction by Promoting Mitochondrial Bioenergetics
    Article Snippet: .. Sample preparation: Tissues were homogenized separately in 400 μL lysis buffer containing RIPA and Extraction Buffer, protease inhibitor, phosphatase inhibitor and 8 M urea using the gentleMACS Dissociator (Miltenyi Biotec). .. Extracted proteins were reduced and alkylated respectively with 10 mM dithiothreitol (Sigma-Aldrich, #D0632) and 15 mM iodoacetamide (Sangon Biotech, #A600539).

    Article Title: Functional analogs of mammalian 4E-BPs reveal a role for TOR in global plant translation.
    Article Snippet: .. 1 g 10-day old 35S::HF-RPL18 seedlings were homogenized by grinding in 2 mL extraction buffer (200 mM Tris-HCl pH9.0, 200 mM KCl, 25mMEGTA pH 8.0, 35mMMgCl2, 0.05%DOC, 0.05%PTE, 0.05%BriJ- 35, 0.05%Triton X-100, 0.05% Igepal, 0.05%Tween 20, 10 mM DTT, 10 mM Mg132, protease inhibitor EDTA-free) at 4 C for 10 to 15 min before centrifugation at 16,000 g for 10 min at 4 C. The clear supernatant was incubated with 50 mL of mMACSTM Anti-Tag MicroBeads (Miltenyi Biotec, DYKDDDDK Isolation Kit: 130-101-591) for 30 min at 4 C and then loaded in a mMACSTM column. ..

    Incubation:

    Article Title: The Arabidopsis homolog of microspherule protein 1 is essential for embryogenesis and interacts with the Myb-like transcription factor DRMY1.
    Article Snippet: The evolutionarily conserved microspherule protein 1 (MCRS1) has diverse functions, ranging from transcriptional regulation to stabilization of microtubule minus ends in acentrosomal spindles in mammals.. A previous study suggested that in the model plant Arabidopsis thaliana, inactivation of an MCRS1 homolog gene led to aborted embryogenesis.. To test whether this lethality was caused solely by sporophytic defects, we used the heterozygous emb1967-1/mcrs1-1 mutant for reciprocal crosses with the wild-type plant and found that the MCRS1 gene was dispensable for the development of both male and female gametophytes.

    Article Title: Functional analogs of mammalian 4E-BPs reveal a role for TOR in global plant translation.
    Article Snippet: .. 1 g 10-day old 35S::HF-RPL18 seedlings were homogenized by grinding in 2 mL extraction buffer (200 mM Tris-HCl pH9.0, 200 mM KCl, 25mMEGTA pH 8.0, 35mMMgCl2, 0.05%DOC, 0.05%PTE, 0.05%BriJ- 35, 0.05%Triton X-100, 0.05% Igepal, 0.05%Tween 20, 10 mM DTT, 10 mM Mg132, protease inhibitor EDTA-free) at 4 C for 10 to 15 min before centrifugation at 16,000 g for 10 min at 4 C. The clear supernatant was incubated with 50 mL of mMACSTM Anti-Tag MicroBeads (Miltenyi Biotec, DYKDDDDK Isolation Kit: 130-101-591) for 30 min at 4 C and then loaded in a mMACSTM column. ..

    Article Title: Functional analogs of mammalian 4E-BPs reveal a role for TOR in global plant translation.
    Article Snippet: .. Myc-4EBP2 IP 1 g 10-day old 4EBP2ox seedlings were homogenized by grinding in 2 mL extraction buffer (50 mM Tris-HCL, pH 7.5; 100 mMNaCL, 5% Glycerol, 0.05% Tween 20) at 4 C for 10 to 15 min before centrifugation at max speed for 10 min at 4 C. The clear supernatant was incubated with 50 mL of c-myc micro-beads (Miltenyi Biotec, mMACSTM c-myc Isolation Kit: 130-091-123) for 30 min at 4 C and then loaded in a mMACSTM column. ..

    Centrifugation:

    Article Title: The Arabidopsis homolog of microspherule protein 1 is essential for embryogenesis and interacts with the Myb-like transcription factor DRMY1.
    Article Snippet: The evolutionarily conserved microspherule protein 1 (MCRS1) has diverse functions, ranging from transcriptional regulation to stabilization of microtubule minus ends in acentrosomal spindles in mammals.. A previous study suggested that in the model plant Arabidopsis thaliana, inactivation of an MCRS1 homolog gene led to aborted embryogenesis.. To test whether this lethality was caused solely by sporophytic defects, we used the heterozygous emb1967-1/mcrs1-1 mutant for reciprocal crosses with the wild-type plant and found that the MCRS1 gene was dispensable for the development of both male and female gametophytes.

    Article Title: Functional analogs of mammalian 4E-BPs reveal a role for TOR in global plant translation.
    Article Snippet: .. 1 g 10-day old 35S::HF-RPL18 seedlings were homogenized by grinding in 2 mL extraction buffer (200 mM Tris-HCl pH9.0, 200 mM KCl, 25mMEGTA pH 8.0, 35mMMgCl2, 0.05%DOC, 0.05%PTE, 0.05%BriJ- 35, 0.05%Triton X-100, 0.05% Igepal, 0.05%Tween 20, 10 mM DTT, 10 mM Mg132, protease inhibitor EDTA-free) at 4 C for 10 to 15 min before centrifugation at 16,000 g for 10 min at 4 C. The clear supernatant was incubated with 50 mL of mMACSTM Anti-Tag MicroBeads (Miltenyi Biotec, DYKDDDDK Isolation Kit: 130-101-591) for 30 min at 4 C and then loaded in a mMACSTM column. ..

    Article Title: Functional analogs of mammalian 4E-BPs reveal a role for TOR in global plant translation.
    Article Snippet: .. Myc-4EBP2 IP 1 g 10-day old 4EBP2ox seedlings were homogenized by grinding in 2 mL extraction buffer (50 mM Tris-HCL, pH 7.5; 100 mMNaCL, 5% Glycerol, 0.05% Tween 20) at 4 C for 10 to 15 min before centrifugation at max speed for 10 min at 4 C. The clear supernatant was incubated with 50 mL of c-myc micro-beads (Miltenyi Biotec, mMACSTM c-myc Isolation Kit: 130-091-123) for 30 min at 4 C and then loaded in a mMACSTM column. ..

    Purification:

    Article Title: The Arabidopsis homolog of microspherule protein 1 is essential for embryogenesis and interacts with the Myb-like transcription factor DRMY1.
    Article Snippet: The evolutionarily conserved microspherule protein 1 (MCRS1) has diverse functions, ranging from transcriptional regulation to stabilization of microtubule minus ends in acentrosomal spindles in mammals.. A previous study suggested that in the model plant Arabidopsis thaliana, inactivation of an MCRS1 homolog gene led to aborted embryogenesis.. To test whether this lethality was caused solely by sporophytic defects, we used the heterozygous emb1967-1/mcrs1-1 mutant for reciprocal crosses with the wild-type plant and found that the MCRS1 gene was dispensable for the development of both male and female gametophytes.

    Magnetic Beads:

    Article Title: The Arabidopsis homolog of microspherule protein 1 is essential for embryogenesis and interacts with the Myb-like transcription factor DRMY1.
    Article Snippet: The evolutionarily conserved microspherule protein 1 (MCRS1) has diverse functions, ranging from transcriptional regulation to stabilization of microtubule minus ends in acentrosomal spindles in mammals.. A previous study suggested that in the model plant Arabidopsis thaliana, inactivation of an MCRS1 homolog gene led to aborted embryogenesis.. To test whether this lethality was caused solely by sporophytic defects, we used the heterozygous emb1967-1/mcrs1-1 mutant for reciprocal crosses with the wild-type plant and found that the MCRS1 gene was dispensable for the development of both male and female gametophytes.

    Isolation:

    Article Title: The Arabidopsis homolog of microspherule protein 1 is essential for embryogenesis and interacts with the Myb-like transcription factor DRMY1.
    Article Snippet: The evolutionarily conserved microspherule protein 1 (MCRS1) has diverse functions, ranging from transcriptional regulation to stabilization of microtubule minus ends in acentrosomal spindles in mammals.. A previous study suggested that in the model plant Arabidopsis thaliana, inactivation of an MCRS1 homolog gene led to aborted embryogenesis.. To test whether this lethality was caused solely by sporophytic defects, we used the heterozygous emb1967-1/mcrs1-1 mutant for reciprocal crosses with the wild-type plant and found that the MCRS1 gene was dispensable for the development of both male and female gametophytes.

    Article Title: Functional analogs of mammalian 4E-BPs reveal a role for TOR in global plant translation.
    Article Snippet: .. 1 g 10-day old 35S::HF-RPL18 seedlings were homogenized by grinding in 2 mL extraction buffer (200 mM Tris-HCl pH9.0, 200 mM KCl, 25mMEGTA pH 8.0, 35mMMgCl2, 0.05%DOC, 0.05%PTE, 0.05%BriJ- 35, 0.05%Triton X-100, 0.05% Igepal, 0.05%Tween 20, 10 mM DTT, 10 mM Mg132, protease inhibitor EDTA-free) at 4 C for 10 to 15 min before centrifugation at 16,000 g for 10 min at 4 C. The clear supernatant was incubated with 50 mL of mMACSTM Anti-Tag MicroBeads (Miltenyi Biotec, DYKDDDDK Isolation Kit: 130-101-591) for 30 min at 4 C and then loaded in a mMACSTM column. ..

    Article Title: Functional analogs of mammalian 4E-BPs reveal a role for TOR in global plant translation.
    Article Snippet: .. Myc-4EBP2 IP 1 g 10-day old 4EBP2ox seedlings were homogenized by grinding in 2 mL extraction buffer (50 mM Tris-HCL, pH 7.5; 100 mMNaCL, 5% Glycerol, 0.05% Tween 20) at 4 C for 10 to 15 min before centrifugation at max speed for 10 min at 4 C. The clear supernatant was incubated with 50 mL of c-myc micro-beads (Miltenyi Biotec, mMACSTM c-myc Isolation Kit: 130-091-123) for 30 min at 4 C and then loaded in a mMACSTM column. ..

    Article Title: Time series single-cell transcriptional atlases reveal cell fate differentiation driven by light in Arabidopsis seedlings
    Article Snippet: .. For single-nucleus data, the shoot tissues of dark-grown seedlings were frozen in liquid nitrogen, added to nuclear extraction buffer, fragmented with a gentleMACS Octo dissociator (Miltenyi Biotec, 130-095-937), and finally filtered and suspended in 300 μl of 10x wash buffer for nuclear isolation . .. The protoplast or nuclear suspension was loaded into Chromium microfluidic chips with v.3.1 chemistry and barcoded with a Chromium Controller (10x Genomics).



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